LOCUS       UGT2B28-pGEc2           6702 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(6180..6309)
                     /note="3'-ITR"
     misc_feature    complement(5878..6092)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    5330..5871
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    4509..4531
                     /note="attB2"
     misc_feature    5309..5314
                     /note="BstB1 site"
     misc_feature    4532..5227
                     /note="Super GFP"
     misc_feature    5228..5278
                     /note="AviTag"
     misc_feature    5279..5302
                     /note="His Tag"
     PCR_frame       3011..4508
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    3011..4486
                     /note="OPTIMIZED UGT2B28 coding region "
     misc_feature    4487..4507
                     /note="TEV"
     vector          join(4517..6702,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..4516
                     /source="UGT2B28 pDONR"
                     /type="Entry Clone"
     source          1..6702
                     /dnas_title="UGT2B28-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGGCCCTGA AGTGGACATC TGTGCTGCTG CTGATCCACC TGGGCTGCTA
     3061 CTTTAGCAGC GGCTCTTGTG GAAAGGTGCT CGTGTGGACA GGCGAGTACA GCCACTGGAT
     3121 GAACATGAAG ACCATCCTGA AAGAACTGGT GCAGCGGGGC CACGAAGTGA CAGTGCTGGC
     3181 CTCTTCTGCC AGCATCCTGT TCGACCCCAA TGACGCCTTC ACACTGAAGC TGGAAGTGTA
     3241 CCCCACCAGC CTGACCAAGA CCGAGTTCGA GAACATCATC ATGCAGCAGG TCAAACGGTG
     3301 GTCCGATATC CAGAAGGACA GCTTCTGGCT GTATTTCAGC CAGGAACAGG AAATCCTCTG
     3361 GGAGTTCCAC GACATCTTCC GGAATTTCTG CAAGGACGTG GTGTCCAACA AGAAAGTGAT
     3421 GAAGAAGCTG CAGGAAAGCA GATTCGACAT TATCTTCGCC GACGCATTCT TCCCATGTGG
     3481 CGAACTGCTG GCCGCCCTGC TGAATATCCC TTTCGTGTAC AGCCTGTGCT TCACCCCCGG
     3541 CTACACCATT GAAAGACACT CTGGCGGCCT GATCTTCCCA CCCAGCTATA TCCCTGTGGT
     3601 CATGAGCAAG CTGAGCGACC AGATGACCTT CATGGAACGC GTGAAGAACA TGATCTACGT
     3661 GCTGTACTTC GACTTCTGGT TTCAGATGTG CGATATGAAG AAATGGGATC AGTTCTACAG
     3721 CGAGGTGCTG GGCAGACCCA CCACCCTGTT TGAGACAATG GGCAAGGCCG ATATCTGGCT
     3781 GATGCGGAAC AGCTGGTCCT TCCAGTTCCC CCACCCCTTT CTGCCCAACA TCGATTTTGT
     3841 GGGCGGACTG CACTGCAAGC CTGCCAAGCC TCTGCCTAAA GAGATGGAAG AGTTCGTCCA
     3901 GTCCAGCGGC GAAAACGGCG TGGTGGTGTT TTCTCTGGGC AGCGTGATCA GCAACATGAC
     3961 CGCCGAGAGG GCCAATGTGA TTGCCACAGC CCTGGCCAAG ATCCCCCAGA AAGTGCTGTG
     4021 GCGCTTCGAC GGAAACAAGC CTGATGCCCT GGGCCTGAAC ACCAGACTGT ACAAGTGGAT
     4081 CCCTCAGAAC GATCTGCTGG GACTGCCCAA GACCAGAGCC TTTATTACCC ACGGCGGAGC
     4141 CAATGGCATC TACGAGGCCA TCTATCACGG CATCCCCATG GTCGGAATCC CCCTGTTTTG
     4201 GGACCAGCCC GACAATATCG CCCACATGAA GGCTAAAGGC GCCGCTGTGC GGCTGGATTT
     4261 TCACACCATG TCTAGCACCG ACCTGCTCAA CGCCCTGAAA ACCGTGATCA ACGACCCCAG
     4321 CTACAAAGAA AACGTCATGA AGCTCAGCAT CATCCAGCAC GATCAGCCCG TGAAACCTCT
     4381 GCACAGAGCC GTGTTCTGGA TCGAGTTCGT GATGTGTCAC AAGGGCGCCA AGCACCTGAG
     4441 AGTGGCCGCT AGAGATCTGA CATGGTTCCA GTACCACAGC CTGGACgaaa acttgtactt
     4501 tcaaggctCC AGCTTTCTTG TACAAAGTGG TATGAGCAAG GGCGAGGAAC TGTTCACCGG
     4561 CGTGGTGCCC ATCCTGGTGG AACTGGATGG CGACGTGAAC GGCCACAAGT TCTCTGTGCG
     4621 GGGAGAGGGC GAAGGCGACG CCACAAATGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC
     4681 CGGCAAGCTG CCCGTGCCTT GGCCTACCCT CGTGACCACA CTGACCTACG GCGTGCAGTG
     4741 CTTCAGCAGA TACCCCGACC ATATGAAGCG GCACGACTTC TTCAAGAGCG CCATGCCCGA
     4801 GGGCTACGTG CAGGAACGGA CCATCAGCTT CAAGGACGAC GGCACCTACA AGACCAGAGC
     4861 CGAAGTGAAG TTCGAGGGCG ACACCCTCGT GAACCGGATC GAGCTGAAGG GCATCGATTT
     4921 CAAAGAGGAC GGCAACATCC TGGGCCACAA GCTGGAGTAC AACTTCAACA GCCACAACGT
     4981 GTACATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA TCCGGCACAA
     5041 CGTGGAAGAT GGCAGCGTGC AGCTGGCCGA CCACTACCAG CAGAACACCC CCATCGGAGA
     5101 TGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGAGCGTGC TGAGCAAGGA
     5161 CCCCAACGAG AAGCGGGACC ACATGGTGCT GCTGGAATTC GTGACCGCCG CTGGCATCAC
     5221 CCACGGCATG AGCGGCCTGA ACGACATCTT CGAGGCCCAG AAAATCGAGT GGCACGAGCA
     5281 CCACCATCAT CACCATCACC ACTGATGATT CGAAAAATAA TAAGGATCCA ATCAACCTCT
     5341 GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT
     5401 ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT
     5461 TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT
     5521 CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT
     5581 TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC
     5641 GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA
     5701 CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC
     5761 CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA
     5821 CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GAGATCTGCC
     5881 TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG
     5941 ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT
     6001 TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG
     6061 GATTGGGAAG ACAATAGCAG GCATGCTGGG GACTCGAGTT AAGGGCGAAT TGCCGATTAG
     6121 GATCTTCCTA GAGCATGGCT ACGTAGATAA GTAGCATGGC GGGTTAATCA TTAACTACAA
     6181 GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC
     6241 CGGGCGACCA AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG
     6301 AGCGCGCAGC CTTAATTAAG AGCTCATGGC GCGCCTAGGC CTTGACGGCC TTCCGCCAAT
     6361 TCGCCCTATA GTGAGTCGTA TTACGTCGCG CTCACTGGCC GTCGTTTTAC AACGTCGTGA
     6421 CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG
     6481 CTGGCGTAAT AGCGAAGAGG CCCGCACCGA AACGCCCTTC CCAACAGTTG CGCAGCCTGA
     6541 ATGGCGAATG GGAGCGCCCT GTAGCGGCCA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT
     6601 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA
     6661 TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AG
//

